Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
نویسندگان
چکیده
منابع مشابه
Spatial control of active CDC-42 during collective migration of hypodermal cells in Caenorhabditis elegans.
Collective epithelial cell migration requires the maintenance of cell-cell junctions while enabling the generation of actin-rich protrusions at the leading edge of migrating cells. Ventral enclosure of Caenorhabditis elegans embryos depends on the collective migration of anterior-positioned leading hypodermal cells towards the ventral midline where they form new junctions with their contralater...
متن کاملMolecular genetics of the Caenorhabditis elegans heterochronic gene lin-14.
We describe a general strategy for the genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical genetic loci within about 100 kb of any gene in Caenorhabditis elegans. We have used this strategy of parallel RFLP mapping to clone the heterochronic gene lin-14, which controls the timing...
متن کاملWnt signaling controls temporal identities of seam cells in Caenorhabditis elegans.
The Wnt signaling pathway regulates multiple aspects of the development of stem cell-like epithelial seam cells in Caenorhabditis elegans, including cell fate specification and symmetric/asymmetric division. In this study, we demonstrate that lit-1, encoding the Nemo-like kinase in the Wnt/beta-catenin asymmetry pathway, plays a role in specifying temporal identities of seam cells. Loss of func...
متن کاملThe Caenorhabditis elegans GATA factor elt-1 is essential for differentiation and maintenance of hypodermal seam cells and for normal locomotion.
The Caenorhabditis elegans GATA transcription factor elt-1 has previously been shown to have a central role in the specification of hypodermal (epidermal) cell fates and acts several cell divisions before the birth of hypodermal cells. Here we report that elt-1 also has essential functions during subsequent development. Reporter gene studies show that elt-1 expression is maintained in lateral s...
متن کاملIdentification of heterochronic mutants in Caenorhabditis elegans. Temporal misexpression of a collagen::green fluorescent protein fusion gene.
The heterochronic genes lin-4, lin-14, lin-28, and lin-29 specify the timing of lateral hypodermal seam cell terminal differentiation in Caenorhabditis elegans. We devised a screen to identify additional genes involved in this developmental timing mechanism based on identification of mutants that exhibit temporal misexpression from the col-19 promoter, a downstream target of the heterochronic g...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3132